This picture was taken by me at the public library in Stuttgart, Germany
May, 2019
Publications
Included are a list of publications relevant to my research, both from my lab (Xie Laboratory, North Carolina State University) and other institutions. My own publications will later be included here.
Artemisinin biosynthesis in Artemisia annua and metabolic engineering: questions, challenges, and perspectives
This review paper from my lab outlines the history of the anti-malarial drug artemisinin, including its mode of action against malaria parasites, its implementation as a treatment, and the elucidation of its biosynthetic pathway. A discussion on genetic breeding of Artemisia annua plants for increased artemisinin production and metabolic engineering strategies (including synthetic techniques) is also included.
A Genome-wide scenario of terpene pathways in self-pollinated Artemisia annua
This paper from my lab outlines the various important regulatory networks, genes, and proteins that function to generate artemisinin and its derivatives. This paper mentions the cytochrome P450 reductase homolog (CPR2) that I am working to characterise.
Cytochrome P450 enzymes: A driving force of plant diterpene diversity
This review paper provides a summary of plant Cytochrome P450 (CYP) enzymes and their significance in plant secondary metabolism. It is worth noting that diterpenes are large molecules that contain 20 carbon atoms and artemisinin is classified as a sesquiterpene, meaning it only has 15 carbon atoms. While some differences therefore may prevail regarding the synthesis of these molecules, the overall function, evolution, and diversity of CYP enzymes remains the same.
A large-scale comparative analysis of affinity, thermodynamics and functional characteristics of interactions of twelve cytochrome P450 isoforms and their redox partners
To get a sense of some of the techniques I hope to include in my own research I have included a paper that details the use of super plasmon resonance (SPR) to address the different kinetic and thermodynamic properties that exist for CYP enzymes and their partner enzymes (such as Cytochrome P450 Reductases)